Black Queen Cell Disease in Honeybees. Effect of a Citric Extract Adsorbed to a Titanium Nanoparticle

Titanium nanoparticles with citrus extracts adsorbed to its structure dissolved in water was used to control this viral disease of bees. Initially, two nuclei of two- and three-frame bees, four well-populated three-frame nuclei with a progenitor queen selected and inseminated to obtain larvae for translarvae, two starter hives consisting of two jumbo hive bodies separated by a queen excluder, two finisher hives with a queen excluder and a queen excluder were used, two finishing hives with a capacity of one and a half jumbo hives (fifteen frames), with a queen excluder placed vertically at the height of the tenth frame acting as a separator between the queen part and the queenless section. 1ST STAGE. A dilution of 1 litre of product in 10,000 litres of water, equivalent to 0.1 ml of product per litre of water. 2nd STAGE A dilution of 1 litre in 200 litres of water, equivalent to 5 ml of product per litre of water. The dilution was sprayed directly to each of the honeycombs with the bees on the surface. Three applications, one every week, the product has a life of 72 hours. After the last application there have been at least 5 translarvae without signs of the disease in the real cells of 10 to 11 days of age and transferred to their nuclei of fertilization, there were deaths of pupae or queens almost to be born but these are fully formed situation or symptom that does not correspond to the disease that concerns us. It is concluded that NANOCYT is effective in controlling black queen cell disease in honey bees.