In-Vitro Antioxidant Activities of Aqueous and Methanol Extracts of Enicostemma littorale Blume
Downloads
The total phenolic and flavonoid contents and antioxidant potential of hot aqueous and methanol extracts of whole plant of Enicostemma littorale Blume was investigated. The percentage yield of hot aqueous extractive value (36.3%) was greater than the percentage yield of hot methanol extractive value (21.8%) and the total phenolic and flavonoid contents in hot methanol extract were found to be higher than in the hot aqueous extract of Enicostemma littorale. Overall results of in-vitro antioxidant activity assays indicated that in comparison to the standard trolox, Enicostemma littorale showed low antioxidant activity in DPPH, ABTS and FRAP methods. Iron chelating activity not found at highest possible concentration of both extracts. Although the total phenolic and flavonoids components were detected in the hot methanol and aqueous extracts, these does not appear to be reported a comparison between the observed in-vitro antioxidant activity and extractive values with total phenolic and flavonoids contents of Enicostemma littorale.
Sons, S. and Lewis, B.A. 2002. Free radical scavenging and antioxidant activity of Caffeic acid amide and Ester, Journal of Agriculture and Food chemistry; 50: pp. 468-472.
Ahmed, E., Arshad, M., and Ahmed, M., saeed, M. and Ishaque, M. 2004. Ethnopharmacological survey of some medicinally important plants of Galliyat areas of NWFP. Pakistan Asian Journal of Plant Sciences; 3: pp. 410-415.
Benzine, I.F.F and Szeto, Y.T. 1999. Total antioxidant capacity of teas by the ferric reducing antioxidant power assay’. J. Agric. Food Chem; 47: pp. 633-636.
Roberta, R., Pellegrini. N., Proteggente. A., Pannala. A., Yang. M. 1999. Antioxidant activity applying an improved ABTS radical cationdepolarization Assay, Free Radical Biol. Med; 26: pp. 1231-1237.
Singleton, V.L., Orthofer, R and Raventos, R.M.L. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaltue reagent, Meth Enzymol; 299: pp. 152-178.
Murukesu Mudaliyar, K.S 1988. MateriaMedica I (Vegetable kingdom). Siddha maruthuvavarrium, Chennai 600106; 4th ed.: pp. 20-607.
Kannusamipillai, S. 1998, Siddha Vaidthiya Patharththa Guna Vilakam, (Moolavarkkam), B. Rathinanajakar & Sons, Chennai; 6th ed.: pp. 09-671.
Nadharni, K. 2002. Indian Materia Medica, Vegetable kingdom, Bombay Popular Prakashan; Vol. I, pp. 485, 1292-1294.
Kirtikar, K.R., Basu, B.D. 2003. Indian Medicinal Plants, Bishen Singh Mahendra Pal Singh, Delhi; Vol. I – V (Vol. III, 2: pp. 1655-1656, 1774-1777).
Vidyadhar, S., Saidulu, M., Gopal, T.K., Chamundeeswari, D., Umamaheswara rao, David Banji. 2010. In Vitro Anthelmintic activity of the whole plant of Enicostemma littoraleby using various extracts, International Journal of Applied Biology and Pharmaceutical Technology; Vol. I, Iss. 3: pp. 1119-1125.
Dassanayake, M.D., Fosberg, F.R. 1981. A Revised Handbook to the Flora of Ceylon. New Delhi: Oxford and IBH Publishing Co.
Lakshmi, P.T.V., Annamalai, A and Ramya, C. 2011. A study on the Genetic Diversity Analysis of a Medicinally potential herb- Enicostemma littorale Biume (Gentianaceae), International Journal of Pharma and Bio Sciences; Vol. 2(4): pp. 238-445.
Singleton, V.L, Orthofer R., Lamuela-Raventos R. M., “Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocaltue reagent”, Meth Enzymol, 299, pp. 152-178.
Badarinath A. V., Mallikarjuna RAo K., Chetty C. M. S.,Ramkanth S, Rajan T. V. S., Gnanaprakash K., 2010.A Review on In-vitro Antioxidant Methods: Comparisons, Correlations and Considerations, International Journal of Pharm Tech Research, vol. 2. no.2, pp. 1276-85.
Blois M. S., 1958. Antioxidant determination by use of stable free radical, Nature, 181, pp. 1199-1200.
Benzine I. F. F., Szeto Y. T., 1999. Total antioxidant capacity of teas by the ferric reducing antioxidant power assay, J. Agric. Food Chem., 47, pp. 633-36.
Pellegrini R. Re, N., Proteggente A., Pannala A., Yang M., 1999. Antioxidant activity applying an improved ABTS radical cationdecolorization Assay, Free Radical Biol. Med, 26,pp. 1231-37.
Carter P., 1971. Spectrophotometric determination of serum iron at the sub-microgram level with a new reagent ferrozine, Annual Biochemistry, 40, pp. 450-58.
Gite V.N., Pokharkar R.D., Chopade V.V., Takate S.B., 2010. Evaluation of Physicochemical Standardization Parameters of Enicostemma axillare, J. Biosci Tech, Vol. 1 (4), 187-190.
Hinneburg I., Dorman H. J. D., Hiltunen R., 2006. Antioxidant activities of extracts from selected culinary herbs and spices, Food Chemistry 97, pp. 122–129.
Nabavi S. M., Ebrahimzadeli M. A., Nabavi S. F., Hamidina A. Bekhradnia A. R., 2008. Determination of antioxidant activity, phenol and flavonoid content of Parrota Persia MEY, Pharmacology online, 2, pp. 560-567.
Yadava S. A., Hakkim L. F., Sathishkumar R. 2011. Antioxidant activity of Withaniasomnifera (L) Dunal by different solvent extraction methods, Journal of Pharmacy Research, vol. 4 (5), pp. 1428-1430.
Jaishree, V., Badami, S. and Bhojraj, S. 2008. In Vitro Antioxidant activity of Enicostemma axillare, Journal of Health Science; 54: pp. 524-528.
Sathishkumar, R., Lakshmi, P.T.V. and Annamalai, A. 2009. Effect of drying treatment on the content of antioxidants in Enicostemma littorale Blume”. Research Journal of Medicinal plant; 3: pp. 93-101.
Azam, S., Hadi, N., Khan N.V., and Hadi, S.W. 2003. Antioxidant and pro oxidant properties of caffeine, theobromine, and xanthene, Medical Science Monitor; 9: pp. 325-330.
Shylesh, B.S and Padikkala, J. 1999. Antioxidant and anti-inflammatory activity of Emilia sonchifolia, Filoterapia; 70: pp. 275-278.
De Sousa, E., Zanatta, L., Seifriz, I., Creczynski-Pasa, T.B., Pizzolatti, M.G., Szpoganicz, B and Silva, F.R. 2004. Hypoglycemic effect and antioxidant potential of kaempferol-3, 7-0-(alpha)-dirhamnoside from Bauhinia of forficate leaves, Journal of Natural Products; 67: pp. 829-832.
Derore SL, Khadabadi SS, Bhagure L, Ghorpade DS, 2008. In vitro antimicrobial and antioxidant studies on Enicostemma axillare (Lam.) Raynal. Leaves. NPR; 7: 409-412.
Sathishkumar, R., Lakshmi, P.T.V. and Annamalai, A. 2010. Comparative analysis of Non-enzymatic and Enzymatic antioxidants of Enicostemma littorale Blume, International Journal of Pharma and Bio Sciences; Vol. 1(2): pp. 1-16.
All Content should be original and unpublished.