Isolation and Selection of some Antagonizing Fungal Strains of Trichoderma And Chaetonium that can Inhibit the Growth of Pathogenic Fungi on Orange Trees
Downloads
Decline disease has been discovered for a long time but infection is more extensively increasing. It is difficult to detect because the disease is originated from roots. Among the causes, soil fungi have been widely determined. Using fungicides is not an effective way to control the disease. In this case, biocontrol with suitable microbial strains is a potential approach. This study aims to investigate in vitro the possibility of using Trichoderma and Chaetonium to control the causing fungi. Two Chaetonium strains and 1 Trichoderma asperellum strain were isolated from diseased-root samples. Six strains of Trichoderma (isolated strains T1, T2, T3, T4, T5, T6) and three strains of Chaetonium (isolated strains C1, C2, C3) showed a reasonably antagonistic ability to Phytophthora, Fusarium, Rhizoctonia. Base on the PCR identification method, 6 strains of Trichoderma was isolated belonging to Trichoderma asperellum species, and 3 strains of Chaetonium belonging to 2 species Chaetomium globosum and Chaetomium cichlids.
Bui Xuan Dong, Nguyen Huy Van, 2000. Fungi in biotechnology, Science and Technology Publishing House, pp. 148-153.
Burgess LW, Summerell BA, Bullock S, Gott KP, Backhouse D (1994) “Laboratory manual for Fusarium research” (Fusarium Research Laboratory, The University of Sydney and The Royal Botanic Gardens: Sydney)
Chaverri P. Samuels G. (2003). Trichoderma/ Hypocrea (Ascomycota, Hypocreales, Hypocreaceae): species with green ascospores. Studies in Mycology, 48: 1–116.
Chet I. (1987). Trichoderma application, mode of action, and potential as a biocontrol agent of soil-borne plant pathogenic fungi in innovative approaches to plant disease control. John Wiley & Sons, Inc.
Cook R. J. (1993). Making greater use of microbial inoculants in agriculture. Annual Review of Phytopathology, 31: 53–80.
Dighton J., White J.F., Oudemans P. (2005). The fungal community; its organization and role in the ecosystem. Third edition, Taylor & Francis, 93–115.
Druzhinina I.S., Kubicek C.P., Komoń-Zelazowska M., Mulaw T.B., Bissett J. (2010). The Trichoderma harzianum demon: complex speciation history resulting in coexistence of hypothetical biological species, recent genospecies, and numerous relict lineages. BMC Evolutionary Biology, 10: 94–107.
El Amraoui B., Biard J., F., Ikbal F., El Wahidi M., Kandil M., El Amraoui M., Fassouane A. (2015). Activity of Haliscosamine against Fusarium oxysporum f.sp. melonis: in vitro and in vivo analysis. Springer Plus, 4: 1–5
Granke LL, Quesada-Ocampo LM, Hausbeck MK. Variation in Phenotypic Characteristics of Phytophthora capsici isolates from a Worldwide Collection. Plant Dis. 2011 Sep;95(9):1080-1088. DOI: 10.1094/PDIS-03-11-0190. PMID: 30732066.
Sneh, Baruch & Jabaji, Suha & Neate, Stephen & Dijst, G.. (1998). Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control. 10.1007/978-94-017-2901-7.
Soytong, K. and Quimio, T.H. (1989), “Antagonism of Chae globosum to the rice blast pathogen, Pyricularia oryzae”, Kasetsart Journal (Natural Science) 23:198-203
All Content should be original and unpublished.