Giloy (Tinospora Cordifolia): In Vitro Evaluation of Antibacterial Properties

Antibiotic resistance has become a global concern and hence, the search for other source of antimicrobials initiated to find a way to control infections in future. The main objective of this paper is to screen Giloy (Tinospora cordifolia) for its antibacterial activity. The stem of Tinospora cordifolia is used to prepare extract for determining it’s in vitro antibacterial activity as per the agar well diffusion method.  In the agar well diffusion method 100μl of 24 hr broth culture of bacteria was aseptically and evenly swabbed on Mueller Hinton agar plates.  Wells of about 8 mm diameter were aseptically cut using sterile cork-borer. 100 μl of   plant extracts   of different concentrations were then placed into the separate wells. The plates   were   incubated   at 37 ^oC   for 24hr. Antimicrobial activity of the giloy was determined by measuring the diameter of zone of inhibition. The methanolic   extract of Tinospora  cordifolia showed 13, 11, 9 and 5 mm  zone of inhibition in S. aureus cultures   by using 100, 75, 50 and  25 mg/ml concentration, respectively while hot water  extract of Tinospora  cordifolia showed 14, 12, 10 and 8 mm zone of inhibition for S. aureus   by using 100, 75,  50 and  25 mg/ml concentration, respectively and the cold   extract of Tinospora  cordifolia showed 10, 8, 5 and 0 mm zone of inhibition for S. aureus   by using 100, 75,  50 and  25mg/ml concentration, respectively. The methanolic   extract of Tinospora  cordifolia indicated 12, 10, 6 and 4 mm zone of inhibition  in cultures of E.coli by using 100, 75, 50 and  25 mg/ml concentration, respectively and the hot water extract of Tinospora  cordifolia showed  16, 14, 12 and 10 mm zone of inhibition   in cultures of E.coli by using 100, 75, 50 and 25mg/ml concentration, respectively. The cold water extract of Tinospora  cordifolia showed 13, 10, 8, and 5 mm  zone of inhibition in cultures of E.coli  by using 100, 75, 50 and  25 mg/ml concentration, respectively. It has been observed that Tinospora  cordifolia showed very promising results as indicated by the zone of inhibition of bacterial culture through agar well diffusion method that varies from few mm to few cm. This study indicates the in-vitro antibacterial effect of Giloy which needs further validation through in-vivo studies.