Evaluation of Three Covid-19 Antigen Assay Versus PCR Detection in Routine Practice
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According to WHO, molecular testing is the "Gold Standard" for the diagnosis of SARS-CoV-2 infection. However, these tests have some limitations in practice (well trained staff, specific equipment requirements, organization of area and time frame for reporting results). Thus, rapid antigen test (RAT) for the detection of one or several SARS-CoV-2 antigens have emerged. We evaluated the analytical performance of 3 RAT used in our laboratory : FREND® Ag COVID-19 (NanoEntek) (FA), STANDARD Q COVID-19 Ag (SD Biosensor) (BA), PANBIO™ COVID-19 Ag RAPID TEST DEVICE (Abbott) (PA) in comparaison to rRT-PCR results. Our study indicates that PA and SA antigen tests have a very good sensitivity to identify infected patients with COVID-19 specifically between in the 0-5 days time-window post onset of symptoms. Nevertheless, one out of three antigen tests (FA) showed very poor clinical performance.
Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill 2020;25.
Van Honacker E, Van Vaerenbergh K, Boel A, De Beenhouwer H, Leroux-Roels I, Cattoir L. Comparison of five SARS-CoV-2 rapid antigen tests in a hospital setting and performance of one antigen assay in routine practice. A useful tool to guide isolation precautions? Journal of Hospital Infection. https://doi.org/10.1016/j.jhin.2021.03.021.
Toverud Landaas E, Larsdatter Storm M , Christophersen Tollånes M, Barlinn R, Bakken Kran AM, Bragstad K, Christensen A, Andreassen T. Diagnostic performance of a SARS-CoV-2 rapid antigen test in a large, Norwegian cohort J Clin Virol. 2021 Apr; 137: 104789.
Lambert-Niclot S, Cuffel A, Le Pape S, Vauloup-Fellous C, Morand-Joubert L, RoqueAfonso A-M, Le Goff J, Delaugerre C, on behalf of the AP-HP/Universities/INSERM COVID-19 Research Collaboration. 2020. Evaluation of a rapid diagnostic assay for detection of SARSCoV-2 antigen in nasopharyngeal swabs. J Clin Microbiol 58:e00977-20.
Fenollar F, Bouam A, Ballouche M, Fuster L, Prudent E, Colson P, Tissot-Dupont H, Million M, Drancourt M, Raoult D, Fournier P-E. 2021. Evaluation of the Panbio COVID-19 rapid antigen detection test device for the screening of patients with COVID-19. J Clin Microbiol 59:e02589-20. https://doi.org/10.1128/JCM .02589-20.
Berger A, Nsoga MTN, Perez-Rodriguez FJ, Aad YA, Sattonnet-Roche P, Gayet-Ageron A, et al. (2021) Diagnostic accuracy of two commercial SARS-CoV-2 antigen-detecting rapid tests at the point of care in community-based testing centers. PLoS ONE 16(3): e0248921.
https://www.cdc.gov/coronavirus/2019-ncov/lab/ resources/antigen-tests-guidelines.html (Updated Dec. 16, 2020).
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