Evaluation of Three Covid-19 Antigen Assay Versus PCR Detection in Routine Practice

Antigenic test, SARS-CoV-2, Analytical performance.

Authors

  • TAGAJDID Mohamed Rida Laboratory of Virology, Center for Virology, Infectious and Tropical Diseases, Mohammed V Military Teaching Hospital, Mohamed V University, Rabat, 10100, Morocco
  • EL KOCHRI Safae Laboratory of Virology, Center for Virology, Infectious and Tropical Diseases, Mohammed V Military Teaching Hospital, Mohamed V University, Rabat, 10100, Morocco
  • ELANNAZ Hicham Laboratory of Virology, Center for Virology, Infectious and Tropical Diseases, Mohammed V Military Teaching Hospital, Mohamed V University, Rabat, 10100, Morocco
  • ABI Rachid Laboratory of Virology, Center for Virology, Infectious and Tropical Diseases, Mohammed V Military Teaching Hospital, Mohamed V University, Rabat, 10100, Morocco
  • AKHOUAD Youssef Center for Virology, Infectious and Tropical Diseases, Mohammed V Military Teaching Hospital, Mohamed V University, Rabat, 10100, Morocco.
  • REGGAD Ahmed Center for Virology, Infectious and Tropical Diseases, Mohammed V Military Teaching Hospital, Mohamed V University, Rabat, 10100, Morocco.
  • KASSMI Zhour Center for Virology, Infectious and Tropical Diseases, Mohammed V Military Teaching Hospital, Mohamed V University, Rabat, 10100, Morocco.
  • ENNIBI Khalid Center for Virology, Infectious and Tropical Diseases, Mohammed V Military Teaching Hospital, Mohamed V University, Rabat, 10100, Morocco.
  • IDRISS LAHLOU Amine Laboratory of Virology, Center for Virology, Infectious and Tropical Diseases, Mohammed V Military Teaching Hospital, Mohamed V University, Rabat, 10100, Morocco.
May 21, 2021

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According to WHO, molecular testing is the "Gold Standard" for the diagnosis of SARS-CoV-2 infection. However, these tests have some limitations in practice (well trained staff, specific equipment requirements, organization of area and time frame for reporting results). Thus, rapid antigen test (RAT) for the detection of one or several SARS-CoV-2 antigens have emerged. We evaluated the analytical performance of 3 RAT used in our laboratory : FREND® Ag COVID-19 (NanoEntek) (FA), STANDARD Q COVID-19 Ag (SD Biosensor) (BA), PANBIO™ COVID-19 Ag RAPID TEST DEVICE (Abbott) (PA) in comparaison to rRT-PCR results. Our study indicates that PA and SA antigen tests have a very good sensitivity to identify infected patients with COVID-19 specifically between in the 0-5 days time-window post onset of symptoms. Nevertheless, one out of three antigen tests (FA) showed very poor clinical performance.