Concentration dependent effects of some commonly used plant extracts on three different tissue systems: An in vitro study
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The present study was designed to find out the concentration dependent comparative in vitro antioxidative /prooxidative activity of three commonly used plant extracts of AI (Azadirachta indica), OS (Ocimum sanctum) and IR (Inula recemose). For this, in-vitro free radicals scavenging assays such as 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), Nitric oxide (NO), reducing power assay and ferrous sulphate (FeSO4) induced lipid peroxidation (LPO) assay in liver and kidney homogenates and in isolated erythrocytes were performed. Ascorbic acid was used as standard antioxidant for all these in vitro assays. Total polyphenols and flavonoid contents were also estimated. Tested plant extracts were found to exhibit dose dependent antioxidant activities. All three plant were observed to be relatively more protective at lower concentrations. Prooxidative or less protective efficacy of AI was observed in different tissue systems at higher concentration used. All three test drugs were found to be anti-peroxidative. However, higher concentration showed decreased efficacy and/or harmful effects.
Significant amount of polyphenols and flavonoids, present in the test drugs supported their antioxidative property.
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All Content should be original and unpublished.
