Isolation and characterization of secondary metabolites from hexane:benzene:acetone (1:1:1) leave extracts of Pithecellobium dulce leaves
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Objective: The present study describes the isolation and characterization of valuable secondary metabolites from the leave of Pithecellobium dulce (P. dulce).
Methods: The dried leaves of P. dulce were collected, dried, grinded and extracted in soxhlet extractor with hexane: benzene: acetone (1:1:1). The same was subjected to qualitative TLC analysis and finally the isolated compounds were identified using IR, 1HNMR, 13C NMR, Mass spectroscopy15 techniques.
Results: The result of the present investigation showed that the compound 2-(3, 4-dihydroxyphenyl)-5,7-dihydroxy-3-[α-Lrhamnopyranosyl-( 1→6)-β-D-glucopyranosyloxy]-4H-chromen-4-one was obtained which is recognized as rutin in methanol fraction. Its melting point is 315-320 OC. Its molecular formula and weight is C27H30O16.
Conclusion: The obtained leave extract is rich source of rutin which is having strong antioxidant power and can be used for
medicinal purpose.
Nigam SK, Gupta RK, Mitra CR. Pithecellobium dulce. I. Isolation and characterization of the constituents of the legume. J
Pharm Sci, 1962; 52: 459-462.
Adinarayana D, Ramachandraiahchetty P. Chemical investigation of some medicinal plants occurring in southern
India. Indian J Chem1985; 24 :453.
Zapesochnaya GG, Yarosh EA, Syanidze NV, Yarosh GI. Flavanoids of the leaves ofPithecellobium dulce. Khim Prir
Soedin 1980; 2: 252-253.
Saxena VK, Singal M. Geinstein 4'-O-alpha-L rhamnopyranoside from Pithecellobium dulce.Fitoterapia 1998; 694: 305-
Niranjan PS, Kazuo K, Zhonghua J, Sukdeb B, Basudeb A, Tamotsu N. A minor acylated triterpenoid saponin from the
seeds of Pithecellobium dulce. J Chem Res 1999; 32: 558-559.
Chandran PGR, Balaji S. Phytochemical investigation and pharmocological studies of the flowers
of Pithecellobium dulce.Ethnobotanical Leaflets 2008; 12: 245-253.
Sugumaran M, Vetrichelvan T, Darline Quine S. Anti-diabetic potential of aqueous and alcoholic leaf extracts
of Pithecellobium dulce. Asian J Res Chem 2009; 2: 83-85.
Nagmoti DM, Kothavade PS, Bulani VD, Gawali NB, Juvekar AR. Antidiabetic and antihyperlipidemic activity
of Pithecellobium dulce (Roxb.) Benth seeds extract in streptozotocin-induced diabetic rats.Eur J Integrative Med 2015; 7:
–273.
Katekhaye DS, Nagmoti DM. α-Glucosidase and α-amylase inhibitory activities ofPithecellobium dulce bark and
leaves.Phytopharmacology2013; 4: 123-130.
Panda S, Jafri M, Kar A, Meheta BK. Thyroid inhibitory, antiperoxidative and hypoglycemic effects of stigmasterol isolated
from Butea monosperma. Fitoterapia 2009; 80: 123-126.
Alma MS, Chopra N, Ali M, Niwa M. Oleanen and sitgmasterol derivatives from Ambroma augusta. Phytochem 1996; 414:
-1200.
Kamboj A, Saluja AK. Isolation of Stigmasterol and β-sitosterol from petroleum ether extract of aerial parts of Ageratum
conyzoides (Asteraceae). Int J Pharm Pharm Sci 2011; 3: 94-96.
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